Top Crop, From a Starter?

[dordelli]

So i'm on my second step of a two step starter needed for an upcoming Barley wine brew. 1st 2L step of the WLP-001 yeast was uneventful with a small krausen and subdued fermentation but the 2nd 4L step was an explosive fermentation with tons of lost yeast out the side of my 5L flask. I had to resort to spooning out quite a bit of krausen to avoid a huge mess on my stirplate but happened to capture a little into a sanitized jar, I don't have much yeast captured, just enough to cover the bottom of the jar, but my question is, can I use this yeast to make a new starter?

 

[Brettomomyces]

Yes you can, just start small and you can work it up to a larger starter using several steps.

 

[dordelli]

Yes you can, just start small and you can work it up to a larger starter using several steps.

How small, 1L or smaller? How will I know once I have enough yeast for a batch?

 

[Brettomomyces]

http://www.mrmalty.com/calc/calc.html

Use Mr Malty, and change the Repitching from Slurry settings to match what you have