if you do not have beakers to secure the vials in the pressure cooker , is there an alternative? Maybe wrap them together a lot of thick tin foil?
When using Dehydrated Agar Powder, is there an exact Agar: DME:Water ratio? I have read several different ratios.
By volume or weight..
ZBrewski said:Well my Cynmar order is looking to be about $75, did I forget anything? I already have a 1L Flask:
11527910 CLEAR GLASS VIALS w/AUTOCLAVABLE CAP, 24ml 12/PK X2 US$17.00
11520035 5 PIECE BEAKER SET, BOROSILICATE GLASS, 50ml-1000ml-PKG 035 US$18.50
15025994 PLASTIC TEST TUBE RACK, WHITE, HOLDS 24 - 25mmTUBES US$4.25
11533371 PYREX RIBBED FUNNEL, SHORT STEM, 65mm##CL US$7.30
CIM89101 DEHYDRATED AGAR POWDER, 4 oz US$18.00
13225101 NICHROME INOCULATING LOOP, 25 GAUGE WIRE, 8" TOT LENGTH
US$1.50
12030895 GLASS ALCOHOL LAMP w/CAP, 4oz, US$3.75
If Sacc is still lurking this thread (or anyone else has tried it)...
Did you ever get around to culturing any Bug Blends? Agar/Dextrose media either by slants, or separating the strains on plates? If so, how did you make starters?
When I remember to lurk. These days I'm usually just hanging out in the chat room on IRC.
So far I have only slanted brett. It slants just like saccharomyces yeast, it just looks quite a bit different -- the colonies grow up with funny looking fractal shapes that are very neat looking.
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