gatewood
Well-Known Member
- Joined
- Dec 29, 2019
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Sup ppl
I've been experimenting with some aging yeast colonies (starters that I've at least used 6 to 7 times), with the purpose of extending the life and reinvigorating such colonies as much as possible. Since I lack some advanced equipment (e.g. microscope), my only tool has been to subject my colonies to different conditions (heat/cold, aerobic/anaerobic, feeding it simple/complex sugars, etc.) and see what keeps them going best.
Currently, I've found that a combination of:
1. Mild cold (10-15°C)
2. Well aerated (aerobic)
3. Complex sugars (fruit wort subjected to moderate heat, so as to avoid denaturing the sugar polymers)
4. About a 1-2 to 10 ratio of sugar/water ratio (to give the yeast some room and avoid osmotic pressure)
5. Complete darkness
Has managed to keep the vitality of some of my colonies, for up to 10 uses (and still going strong).
So my 2 questions are these:
1. You know of any process that could potentially keep a yeast colony going indefinitely?
2. Is that an optimal thing to do?
I've been experimenting with some aging yeast colonies (starters that I've at least used 6 to 7 times), with the purpose of extending the life and reinvigorating such colonies as much as possible. Since I lack some advanced equipment (e.g. microscope), my only tool has been to subject my colonies to different conditions (heat/cold, aerobic/anaerobic, feeding it simple/complex sugars, etc.) and see what keeps them going best.
Currently, I've found that a combination of:
1. Mild cold (10-15°C)
2. Well aerated (aerobic)
3. Complex sugars (fruit wort subjected to moderate heat, so as to avoid denaturing the sugar polymers)
4. About a 1-2 to 10 ratio of sugar/water ratio (to give the yeast some room and avoid osmotic pressure)
5. Complete darkness
Has managed to keep the vitality of some of my colonies, for up to 10 uses (and still going strong).
So my 2 questions are these:
1. You know of any process that could potentially keep a yeast colony going indefinitely?
2. Is that an optimal thing to do?