AntDoctor
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- Sep 11, 2020
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I wanted to start slanting yeast, and I work in a lab, so I have access to a real industry level autoclave. I just made my first batch of slants tonight, using 90ml water, 9g of DME, 1.8g agar. (2% agar, 10% DME)
I boiled and dissolved the agar with the DME, poured it into some upright culture tubes, loosely topped them with the tubes' caps, the put the bad boys in the autoclave for a "liquid 30" cycle.
But when I removed them from the machine and hour later, the clearish liquid had precipitated material in it. Think the appearance of flocculating yeast, although there definitely isn't any.
What's up with this? How did it happen and how do I avoid it in the future? Did the DME somehow... undissolve and caramelize?? Very confused...
I boiled and dissolved the agar with the DME, poured it into some upright culture tubes, loosely topped them with the tubes' caps, the put the bad boys in the autoclave for a "liquid 30" cycle.
But when I removed them from the machine and hour later, the clearish liquid had precipitated material in it. Think the appearance of flocculating yeast, although there definitely isn't any.
What's up with this? How did it happen and how do I avoid it in the future? Did the DME somehow... undissolve and caramelize?? Very confused...