kidneythief
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I've always wondered how many viable yeast cells there actually are in an 11.5g sachet of yeast (SafAle US-05 in this case). I am soon getting access to some basic lab equipment so I was hoping to learn how one would go about taking a cell count of a fresh, unused packet or brick of yeast.
Would I need to make a starter and take a sample from the resulting slurry or could I simply rehydrate with distilled water (using the packet recommendation of 1:10 yeast/water ratio?) and take a sample from there for the hemocytometer? Also, will methylene blue effectively stain a first generation batch of yeast cells (I've read cell walls may be thicker in first gen)?
Any information would be much appreciated! Cheers
Would I need to make a starter and take a sample from the resulting slurry or could I simply rehydrate with distilled water (using the packet recommendation of 1:10 yeast/water ratio?) and take a sample from there for the hemocytometer? Also, will methylene blue effectively stain a first generation batch of yeast cells (I've read cell walls may be thicker in first gen)?
Any information would be much appreciated! Cheers