I’m on my 16th batch at Kolsch over the last 2 years I think. I’ve used 2565, wlpo29, Dieter, 1007, Gigayeast Kolsch GY021, 2575, 003, Conan, etc. Basically anything and everything. Latest batch used the East Coast Yeast Kolschbier strain and it’s by far the best. It’s supposedly and old Brewtek strain that Al Buck got from a yeast bank. There’s no other commercial equivalent to it.
It has the wine like character of 2565 but flocculation that’s even better than wlp029 or the equivalents from all the other yeast banks.
Up until this my favorite yeast was actually Conan fermented at 56 with a lager pitch rate. Fermented out in 4 days even at 56. Touch of sulfur from the low temp fermentation but insanely clean. Way better than the wlp029 derived offerings.
Kolsch is all about semantics. The conditioning is what’s key. Slow cooling, long cold conditioning, natural carbonation, etc. These are the steps to focus on in order to make truly great Kolsch (provides you’ve got the obvious water profile, mash schedule, and fermentation temp dialed). Sadly there’s probably only a handful of breweries in the US that care to take the time to do it right.