I was reading on BeerSmith about how the larger starting volume of a yeast starter, a sort of "law of diminishing returns" kicks in, so it's harder to get a starter's cell count to, say, 500 billion because it'd require too much starting liquid. The article--and elsewhere--mentioned doing successive step batches, instead, but gave no explanation; nor have I found any with a bit of casual searching. I therefore assume it's simply making successive starters using the yeast from prior ones, e.g. letting a starter's yeast drop out, collect it, then use it for the next starter?
So here's what I'm thinking...does it sound viable to achieve a 500B cell count?
1. In a 3000ml flask, add 1 Wyeast smack pack and 1L of DME+water
2 When done growing, add another 1L of DME+water for total of 2L
I was trying to use 1 online calculator and can't tell if I used it right, but it seems that the 2nd starter should end up at a little over 5B cell count. If I misused it, perhaps I need to do a 3rd starter instead of just 2?
Thoughts? Advice? (I'm not over-thinking this, am I?)
So here's what I'm thinking...does it sound viable to achieve a 500B cell count?
1. In a 3000ml flask, add 1 Wyeast smack pack and 1L of DME+water
2 When done growing, add another 1L of DME+water for total of 2L
I was trying to use 1 online calculator and can't tell if I used it right, but it seems that the 2nd starter should end up at a little over 5B cell count. If I misused it, perhaps I need to do a 3rd starter instead of just 2?
Thoughts? Advice? (I'm not over-thinking this, am I?)