I'll add to this and say that anything targeting 12% and higher should be considered as mandatory to use oxygen through a .5 micron stone. You want something in the area of 8-10 PPM of O2 in your must.
Method of Aeration for 5 gallons | Oxygen PPM |
Shaking for 5 minutes | 2.71 PPM |
Pure 02 for 30 seconds | 5.12 PPM |
Pure O2 for 60 seconds | 9.2 PPM |
Pure O2 for 120 seconds | 14.08 PPM |
The above O2 numbers are with ½ micron aeration stone at 75F. This is from test data by White Labs.
If harvesting yeast for use again, higher O2 levels are desired as low O2 levels will increase the lag phase.
Repeat 12-18 hours after yeast pitch. This will increase fermentation speed and yeast attenuation. This second dose can speed fermentation by as much as 33%.