Lot's of details here, if you don't want to read all this then at least skip to the bottom for my question.
The Yeast is:
I wanted to brew on the 9th but my ADHD got in the way, but I had built up a starter as follows:
I did these three starter steps before I punched any numbers into this calculator. When I realized I wasn't going to brew, I punched the numbers in to see how many cells I made.
Looking at the numbers, I realized that those second and third starter steps were a waste of time due to the low growth factor, so I ordered a 2L flask.
So, when my brew day didn't happen and It was time for my 7days on of 12° night shifts I put my starter flask in the fridge and left it for a week.
Yesterday morning I put the yeast into my new 2L flask and started it up again on the stir plate, and then into the fridge last night.
This morning I decanted and did another 2L starter step, which is still fermenting strongly as I type this right now.
So, I'm thinking I will have plenty of cells to pitch, along with a lot of yeast to drop into my 30% glycerin vials to put in the deep freeze.
Ok, here is my question.
Above it says to pitch 0.646L and harvest 1.354L, I'm planning on cold crashing tonight so I can decant before pitching. So I'm guessing tonight I simply pour off about 0.7L into another flask for pitching tomorrow and then split what is left between a couple of quart mason jars to make the yeast collection easier for my glycerin vials?
Anybody see any problems with my plan or process?
The Yeast is:
I wanted to brew on the 9th but my ADHD got in the way, but I had built up a starter as follows:
I did these three starter steps before I punched any numbers into this calculator. When I realized I wasn't going to brew, I punched the numbers in to see how many cells I made.
Looking at the numbers, I realized that those second and third starter steps were a waste of time due to the low growth factor, so I ordered a 2L flask.
So, when my brew day didn't happen and It was time for my 7days on of 12° night shifts I put my starter flask in the fridge and left it for a week.
Yesterday morning I put the yeast into my new 2L flask and started it up again on the stir plate, and then into the fridge last night.
This morning I decanted and did another 2L starter step, which is still fermenting strongly as I type this right now.
So, I'm thinking I will have plenty of cells to pitch, along with a lot of yeast to drop into my 30% glycerin vials to put in the deep freeze.
Ok, here is my question.
Above it says to pitch 0.646L and harvest 1.354L, I'm planning on cold crashing tonight so I can decant before pitching. So I'm guessing tonight I simply pour off about 0.7L into another flask for pitching tomorrow and then split what is left between a couple of quart mason jars to make the yeast collection easier for my glycerin vials?
Anybody see any problems with my plan or process?