StMarcos
Well-Known Member
Just a data point for all you bankers out there:
I did 2 cultures, stepped up from wyeast packs, into autoclaved starters. 2308 and 1098. After the ferment, I mized 5050 glycerine to slurry. Mixed once in the tubes, and froze immediately to -20F. After a month or two, neither culture woke up well. I added a bit or fresh wort to the tubes, after decanting the glycerine portion, and neither of the cultures really woke up. They were barely fermenting 6 days after the wort addition, and this was only noticeable by shaking the tubes and noting the co2 release. So, this could have been a contaminate I introduced, or just terrible viability. So, the viability was either terrible or zero. Either way it didn't work. Not sure if it was from too much glycerine, or the rapid freeze. It was enough glycerine that the solution was still a liquid after freezing to -20F.
Non-frost-free freezer, and the tubes were inside a small cooler inside with blueice packs.
I might try this again. From what I've read, I should use less glycerine, as well as let the culture sit with the glycerine present for some time (room T and/or fridge T?). Maybe I should try both, and each without the other, as a sort of experiment.
The yeast should have been extremely healthy going into the tubes, so it must have been my procedure/process....
I did 2 cultures, stepped up from wyeast packs, into autoclaved starters. 2308 and 1098. After the ferment, I mized 5050 glycerine to slurry. Mixed once in the tubes, and froze immediately to -20F. After a month or two, neither culture woke up well. I added a bit or fresh wort to the tubes, after decanting the glycerine portion, and neither of the cultures really woke up. They were barely fermenting 6 days after the wort addition, and this was only noticeable by shaking the tubes and noting the co2 release. So, this could have been a contaminate I introduced, or just terrible viability. So, the viability was either terrible or zero. Either way it didn't work. Not sure if it was from too much glycerine, or the rapid freeze. It was enough glycerine that the solution was still a liquid after freezing to -20F.
Non-frost-free freezer, and the tubes were inside a small cooler inside with blueice packs.
I might try this again. From what I've read, I should use less glycerine, as well as let the culture sit with the glycerine present for some time (room T and/or fridge T?). Maybe I should try both, and each without the other, as a sort of experiment.
The yeast should have been extremely healthy going into the tubes, so it must have been my procedure/process....