dstar26t
If it's worth doing, it's worth overdoing
In the Yeast book, it says to use a .5% solution of sulfuric acid to combat flocculation when looking at a sample under a microscope. I'm typically looking at how a starter is doing on the stir plate after 24 hours so my dilution is usually around 5:1. Some strains will still clump so I'm wondering if a stronger sulfuric acid solution is necessary. Also, I've come across some info lately claiming that cells don't retain the dye for checking viability when the diluent is an acid solution. Is this true? If so, what acid concentration is too high for checking viability?
Nate
Nate