building up yeast from a plate

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LouBrew13

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I have a plate of 3 different yeast on the same plate. All were scratched and grown by a friend that knows how to do this stuff in a lab. I've read directions and how do I begin with 10ml then 10x then 10x step ups from a pin?

Can I make my normal starter volume of dme and start stepping this up or would the wort get funky in the interim?

Any easy ways to begin this?
 

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