Recent content by Proteinguy1995

Hey guys, I just started to ferment a new hefe batch 2 days ago with w3068 and am noticing a few issues. It is a small 2.5 gal batch. The OG for the batch was 1.045, and I purposely underpitched (using half the pack, and assuming some loss of viability). The smack pack was swelling properly...

I imagine it would be ok as long as the probiotic can survive the conditions of the beer. It would also be tricky if you are performing natural carbonation with sugar. The probiotic bacteria would likely use some of that sugar, adding off flavours from unwanted metabolic products. I'm sure...

@Dland - Thanks for the response, I for sure would not let anyone drink it till I had one myself. I just found some literature stating its 95% stable in acidic medium after 2 weeks, they didn't look far after. @Silver_Is_Money I was taking doxycycline, 100mg a day for 2 months. I had no...

@bovine_OB - Agreed, and understood from the get go. I am under the assumption that no infectious resistant strains would be present/produced under other conditions in the Wort (low oxygen, less than optimal growing temperature, low pH, high alpha acid content, low sugar post fermentation)...

So far I have found that there is no significant metabolism and no metabolites have been found in man, however nothing on yeast metabolism (for doxycycline). However I assume this is the same, being a less complex organism. Any thoughts? Agwul, N.K., MacGowan, A. (2006) Pharmacokinetics and...

@Silver_Is_Money - Trying to find out what are its possible metabolites/by products. anhydro-4-epitetracycline is not formed however, so no nephrotoxicity from that at least. Only 60mg was used in the whole batch, and the maximum recommended dose is 300-mg daily. I'm not sure if there is too...

@TechFanMD - I already am aware that the batch is likely sterile enough from boiling + high acid content from the hops. My brew methods are a little unsanitary at the moment due to lack of equipment. I racked the primary over to secondary the other day and let the batch be exposed to air for a...

Hey guys, I am a graduate student in biochemistry and do a lot of protein expression/purification using E.Coli, and P.Pastoris (similar strain of yeast to S.Cerevisiae). Generally I use ampicillin in my yeast starters to limit bacterial growth in the initial stages (yeast strain has AMP...