Recent content by onlyhere4science

Will do! Thanks mate.

I was really just here to see if others had tested it… but of course I’d be happy to sit down and answer these personal questions that pertain to my own career situation and curiosity in extensive detail for you to ruminate on and apply to the broader discussion we were having on why there isn’t...

That’s not going to get a paper published, but sure…

NP! The volume of the props produced more cells/mL than needed to meet the pitch rate target of the fermenters/jars. From my understanding of the methods section, they withdrew the volume of cells from each prop required to hit the fermenter pitch target, spun the cells down to get rid of the...

"I could be wrong" nah ur definitely right - it's totes crazy there hasn't been another literature article published yet on this very specific topic which all labs and all brewers are paying close extremely close attention to. I assume you read the journal article immediately when it came out...

They do kinda mention this. I def think components of yeast extract that were non-nitrogen likely contributed positively. Def something future research could build on. While they say they interpreted results based on nitrogen (as that's what the recipes were based on), anyone could replicate the...

Need to remember statistics here... The paper shows no statistically significant difference in attenuation between harvested yeast and yeast propagated in ANY wort gravity that included nitrogen to achieve a C:N at 100. Harvested yeast did not perform statistically better, with the exception...

Anytime dude. Agreed on your thoughts on best case scenario. Paper shows results with a good range of OGs with and without nitrogen. It’s my understanding that adding nitrogen in the prop media regardless of OG had a benefit - last graph shows that attenuation was improved for all OG when...

A fair question for sure. Seems like there was still p good growth in the 2P recipe, but certainly lower than an 8P recipe from the example provided in the table above. I think the quote from the paper below is interesting but def applies more to commercial brewers: The total cell count in low...

If you do what you've always done, you'll get what you've always got. Seems like a common mindset in home brews and commercial brewers, which is fine. No one can argue there is anything wrong with respectable rates and respectable attenuations. On the other hand, there are those that may see the...

You're right - you don't make as many cells by volume with a lower P media at least with the conditions used in the paper. So yes...you make more physiologically shitty cells with high OG. ;) Just for data sake, actual numbers numbers from the paper are in the Supporting Info. If we compare H5...

Maria Moutsoglou addresses the cost for inclusion of yeast extract for nitrogen vs. using full strength wort for prop media here. Sounds like because you're using less DME/wort sugars, it's more cost-effective to produce cells with low OG/high nitrogen vs. standard wort. So the cost comments...