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  1. AntDoctor

    Laboratory autoclave created brown precipitate in DME agar media

    I wanted to start slanting yeast, and I work in a lab, so I have access to a real industry level autoclave. I just made my first batch of slants tonight, using 90ml water, 9g of DME, 1.8g agar. (2% agar, 10% DME) I boiled and dissolved the agar with the DME, poured it into some upright culture...
  2. jester5120

    Plating Yeast & Selecting Colonies

    I've seen several articles on this site about the proper way to slant yeast but haven't found one that shows the process of streaking yeast onto a plate and selecting individual colonies. This is the method I use. It's not flawless and it certainly has room for improvement but it's cheap and...
  3. jester5120

    Plating Yeast & Selecting Colonies

    I've seen several articles on this site about the proper way to slant yeast but haven't found one that shows the process of streaking yeast onto a plate and selecting individual colonies. This is the method I use. It's not flawless and it certainly has room for improvement but it's cheap and...
  4. B

    Questions about juice efficiency

    Hello HBT community, This site and its community is fantastic and has been a staple in my research when brewing and vinting, so thank you all! I looked pretty hard to find info before posting, but I haven’t had much luck. I’m seeking any intel you folks might have on the following: What is...
  5. Eric Carbobiach

    Agar doesn't set

    Hello, friends! I'm having problems with agar. Here in Brazil, lab grade is too expensive, so i'm using food grade, from health store. But only became gel, doesnt firm enought to use the loop. So i tried another store. Same problem. Tried more concentrated, without success. My procedure: I...
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