Viability declines pretty slowly in the fridge.
Yeah, I'm starting to think the viability loss in many of the yeast starter calculators is WAY over-estimating actual loss. I had a vial that was in the fridge for almost a year and yeastcalc.com was telling me I had essentially zero living cells left. I tried building up a starter anyways and, short of doing a cell count, it was pretty obvious there were a bunch of healthy, viable yeast in there.
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